Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hydrogel particle-based protein display enabled by particle-templated emulsification.

RSC advances·2025

Same author

Deciphering the mechanism of hydrogen peroxide formation in ultrasound-mediated water-in-oil microdroplets.

Chemical science·2025

Same author

Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics.

Gels (Basel, Switzerland)·2024

Same author

Advances in Single-Cell Printing.

Micromachines·2022

Same author

Immobilization of Proteins of Cell Extract to Hydrogel Networks Enhances the Longevity of Cell-Free Protein Synthesis and Supports Gene Networks.

ACS synthetic biology·2021

Same author

Tough hybrid microgel-reinforced hydrogels dependent on the size and modulus of the microgels.

Soft matter·2020

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026

Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026

Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 14, 2025

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

Surface modification for improving immunoassay sensitivity.

Xiaohu Zhou¹, Bo Zheng¹

¹Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen 518132, China. bozheng@szbl.ac.cn.

|January 13, 2023

Summary

This summary is machine-generated.

Surface modifications enhance immunoassay sensitivity by reducing background noise and amplifying signals. Strategies include antifouling coatings and nanostructured surfaces for improved biomarker detection.

More Related Videos

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Related Experiment Videos

Last Updated: Aug 14, 2025

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Area of Science:

Biomedical Engineering
Analytical Chemistry
Materials Science

Background:

Immunoassays are crucial for biomarker discovery, drug development, and clinical diagnostics.
Increasing demand for high-sensitivity immunoassays to detect low-abundance analytes.
Surface quality is critical for immunoassay performance.

Purpose of the Study:

To review recent advancements in surface modification strategies for enhancing immunoassay sensitivity.
To categorize and discuss techniques for reducing background noise and amplifying detection signals.

Main Methods:

Review of antifouling coatings: hydrophilic polymers (self-assembled monomers, polymer brushes, hydrogels) and perfluorinated surfaces.
Review of nanostructured surfaces: nanoparticle functionalization, 2D nanoarrays, and 2D nanomaterial coatings.
Discussion of surface modifications for digital immunoassays.

Main Results:

Antifouling coatings effectively minimize nonspecific binding and background noise.
Nanostructured surfaces amplify specific detection signals, boosting sensitivity.
Specific surface techniques are being developed for digital immunoassay platforms.

Conclusions:

Surface modification is key to advancing immunoassay sensitivity.
Continued innovation in antifouling and nanostructured surfaces is essential.
Future research should address challenges and explore new perspectives in surface engineering for immunoassays.