Related Experiment Video
Updated: Jun 24, 2026
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Published on: June 1, 2014
High throughput assays for analyzing transcription factors
Xianqiang Li1, Xin Jiang, Takuro Yaoi
1Panomics Inc., Fremont, CA 94555, USA. jasonli@panomics.com
Abstract:
Transcription factors are a group of proteins that modulate the expression of genes involved in many biological processes, such as cell growth and differentiation. Alterations in transcription factor function are associated with many human diseases, and therefore these proteins are attractive potential drug targets. A key issue in the development of such therapeutics is the generation of effective tools that can be used for high throughput discovery of the critical transcription factors involved in human diseases, and the measurement of their activities in a variety of disease or compound-treated samples. Here, a number of innovative arrays and 96-well format assays for profiling and measuring the activities of transcription factors will be discussed.
Insights
New tools are available for measuring transcription factor activity, aiding in the discovery of disease-related factors and potential drug targets for human diseases.
Area of Science:
- Molecular Biology
- Genetics
- Drug Discovery
Background:
- Transcription factors regulate gene expression in vital biological processes like cell growth and differentiation.
- Dysfunctional transcription factors are implicated in numerous human diseases, making them promising therapeutic targets.
- Effective tools are needed for high-throughput screening to identify critical transcription factors in diseases and assess their activity.
Purpose of the Study:
- To discuss innovative arrays and 96-well format assays for profiling transcription factor activities.
- To present methods for measuring transcription factor activity in disease and compound-treated samples.
- To highlight advancements in tools for drug discovery targeting transcription factors.
Main Methods:
- Development of novel array-based platforms.
- Implementation of 96-well format assays.
- Assays designed for high-throughput screening and activity measurement.
Main Results:
- The study discusses the development and application of innovative tools for transcription factor analysis.
- These tools enable the profiling and measurement of transcription factor activities.
- The methods are suitable for diverse disease and compound-treated sample types.
Conclusions:
- Innovative arrays and assays provide effective means for studying transcription factors.
- These tools facilitate the discovery of disease-critical transcription factors.
- Advancements in assay technology support therapeutic development targeting transcription factors.
More Related Videos
12:54Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
11:32Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
Related Concept Videos
02:16Transcription Factors
02:36Chromatin Immunoprecipitation- ChIP
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...