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Updated: Aug 7, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein

Anne Kerres1, Visnja Jakopec, Christoph Beuter

  • 1Lehrstuhl für funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine Universität, 40225 Düsseldorf, Germany.

Molecular Biology of the Cell
|July 21, 2006
PubMed

Insights

The Sim4 complex in fission yeast is crucial for kinetochore assembly. Researchers identified Fta2 as a key protein interacting with Mal2, essential for chromosome attachment and Sim4 complex function.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The Sim4 complex is vital for kinetochore assembly in fission yeast.
  • It influences CENP-A and DASH complex localization at the kinetochore.

Purpose of the Study:

  • To identify novel proteins interacting with Sim4 complex components.
  • To elucidate the functional roles of Mal2 and Fta2 in kinetochore function.

Main Methods:

  • Multicopy suppressor analysis was used to identify Fta2.
  • Genetic interactions and protein localization studies were performed.

Main Results:

  • Fta2 was identified as an essential kinetochore protein required for bipolar chromosome attachment.
  • Mal2 and Fta2 exhibit interdependent kinetochore localization and overlapping functions.
  • Fta2 localization is dependent on the Sim4 complex but sensitive to Spc7 levels.

Conclusions:

  • Fta2 and Mal2 form a functional subgroup within the Sim4 complex.
  • The Sim4 complex assembly appears hierarchical, with Fta2's localization preceding its dependence on Spc7.
  • These findings reveal functional specialization within the Sim4 complex and its hierarchical assembly.

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