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Updated: Aug 6, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Development of a standardized analysis strategy for basic drugs using ion-pair extraction and high-performance liquid
1Farmaceutisch Instituut, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussel, Belgium.
Abstract:
The first of a series of papers on the development of a standardized analysis strategy for basic drugs explains the possible advantages and philosophy of the strategy. The scheme uses ion-pair extraction with direct injection of the extracts into an HPLC system emanating, from two previously-selected systems. The extraction efficiency of sodium-n-octylsulphate as the ion-pairing reagent is compared with that of di(2-ethylhexyl) phosphoric acid (previously shown to be generally applicable to the extraction of basic drugs). Direct injection of the ion-pair extracts into an HPLC system is possible because retention behaviour is independent of whether the basic drugs are injected as an ion-pair or as a base.
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