Related Experiment Video
Updated: Aug 6, 2026
Laser-Induced Chronic Ocular Hypertension Model on SD Rats
Published on: December 4, 2007
Effects of laser therapy in CO2 laser wounds in rats
Rita Adriana Oliveira Grbavac1, Elaine Bauer Veeck, Jean-Pierre Bernard
1Department of Stomatology and Oral Surgery, University of Geneva, Geneva, Switzerland. Rita.Oliveira-Grbavac@medecine.unige.ch
Objective:
The aim of this study was to assess the effects of laser therapy and its possible dose dependency on the healing of CO2 laser surgical wounds.
Background Data:
Several reports from our group and others have indicated that light therapies may improve healing, depending on wavelength, dose, intensity of the light, and both local and systemic conditions.
Methods:
Circular surgical wounds were created on the dorsum of Wistar rats, which were separated into three groups (A, B, and C). Group A acted as control and had no additional treatment. Groups B and C were irradiated with GaAlAs lambda685-nm laser light, either with 20 J/cm2 (Group B) or 40 J/cm2 (Group C). The animals were humanely killed at the end of the experimental period; specimens were taken and routinely processed to wax and stained with Hematoxylin and Eosin, Sirius Red, and alpha-Smooth Muscle Actin (alphaSMA).
Results:
Laser-irradiated groups showed a healing process characterized by a more prominent fibroblastic proliferation, with young fibroblasts actively producing collagen; no myofibroblasts were found. No statistically significant differences were observed when the different doses were compared.
Conclusion:
It may be concluded that, using this methodology, laser therapy has a positive effect in wound healing produced by CO2 laser, and the dose has no influence on the treatment.
