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Updated: Aug 6, 2026
Bacteriophage Removal from Infected Salmonella Cultures
Published on: June 28, 2024
Factors affecting iron sulfide-enhanced bacteriophage plaque assays in Salmonella
1United States Department of Agriculture, Agricultural Research Service, Waste Management and Forage Research Unit, Mississippi State, Mississippi 39762-5367, USA. mmclaughlin@ars.usda.gov <mmclaughlin@ars.usda.gov>
Abstract:
Reaction of ferric ions with hydrogen sulfide (H(2)S) enhances contrast of phage plaques in H(2)S+ Salmonella, but contrast diminishes in weak H(2)S+ strains. H(2)S was affected by concentrations of peptones, glucose, ferric ammonium citrate (FAC) and sodium thiosulfate (ST), and by FAC:ST ratio, temperature, pH, air, and host strain. Increasing peptone levels was most important for improving contrast in weak H(2)S+ strains.
