Related Experiment Video
Updated: Aug 11, 2026
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
Gene disruption in mammals
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Current Opinion in Genetics & Development
|August 1, 1991
Abstract:
In the past year, the first phenotypes have been reported for mutations targeted to developmentally relevant genes by homologous recombination in embryonic stem cells. The results indicate that the genetic circuitry of mammalian development is complex and will require more sophisticated analysis than simple gene disruption. Improvements in the technology of targeted mutagenesis may assist in such analysis.
Related Concept Videos
00:53Transgenic Organisms
Overview
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.