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Extended focus depth for Fourier domain optical coherence microscopy.

R A Leitgeb1, M Villiger, A H Bachmann

  • 1Laboratoire d'Optique Biomedicale, Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne, Switzerland. rainerleitgeb@epfl.ch

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Summary

We developed a new Fourier domain optical coherence microscopy detection scheme. It achieves high transverse resolution over an extended focal range, improving imaging capabilities.

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Area of Science:

  • Biomedical optics
  • Microscopy techniques
  • Optical imaging

Background:

  • Fourier domain optical coherence microscopy (FD-OCM) is a powerful imaging technique.
  • Achieving high transverse resolution over an extended axial range remains a challenge in FD-OCM.

Purpose of the Study:

  • To introduce and validate a novel detection scheme for FD-OCM.
  • To enhance transverse resolution and extend the usable focal range in FD-OCM.

Main Methods:

  • Implementation of a new detection scheme within the Fourier domain optical coherence microscopy framework.
  • Utilizing a broad-bandwidth Ti:sapphire laser source.
  • Experimental verification of resolution and sensitivity.

Main Results:

  • Achieved nearly constant transverse resolution of approximately 1.5 micrometers.
  • Demonstrated this resolution over an axially extended focal range of 200 micrometers.
  • Verified a maximum sensitivity of 105 dB and an axial resolution of 3 micrometers in air.

Conclusions:

  • The novel detection scheme significantly improves transverse resolution in FD-OCM.
  • The extended focal range broadens the applicability of FD-OCM for various biological samples.
  • This advancement offers enhanced capabilities for high-resolution microscopic imaging.