Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.8K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.8K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

907
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
907
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Positron Emission Tomography01:29

Positron Emission Tomography

6.7K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
6.7K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical coherence tomography.

Nature reviews. Methods primers·2023
Same author

Evaluation and review of body fluids saliva, sweat and tear compared to biochemical hydration assessment markers within blood and urine.

European journal of clinical nutrition·2017
Same author

Optimizing modulation frequency for structured illumination in a fiber-optic microendoscope to image nuclear morphometry in columnar epithelium.

Biomedical optics express·2015
Same author

Optical Coherence Tomographic Imaging of Human Tissue at 1.55 μm and 1.81 μm Using Er- and Tm-Doped Fiber Sources.

Journal of biomedical optics·2012
Same author

Flexible transbronchial optical frequency domain imaging smart needle for biopsy guidance.

Biomedical optics express·2012
Same author

Method of concentration of power in materials for x-ray amplification.

Applied optics·2010
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Dec 21, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.8K

Automated noise estimation in polarization-sensitive optical coherence tomography.

P A Keahey, B E Bouma, M Villiger

    Optics Letters
    |May 16, 2020
    PubMed
    Summary
    This summary is machine-generated.

    Accurately determining the signal noise floor is crucial for polarization-sensitive optical coherence tomography (PS-OCT). This study introduces an automated method using polarization uniformity and speckle statistics for reliable noise floor estimation in PS-OCT systems.

    More Related Videos

    Doppler Optical Coherence Tomography of Retinal Circulation
    10:46

    Doppler Optical Coherence Tomography of Retinal Circulation

    Published on: September 18, 2012

    19.1K
    Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
    04:51

    Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

    Published on: March 1, 2024

    1.3K

    Related Experiment Videos

    Last Updated: Dec 21, 2025

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
    11:21

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

    Published on: January 15, 2013

    11.8K
    Doppler Optical Coherence Tomography of Retinal Circulation
    10:46

    Doppler Optical Coherence Tomography of Retinal Circulation

    Published on: September 18, 2012

    19.1K
    Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
    04:51

    Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

    Published on: March 1, 2024

    1.3K

    Area of Science:

    • Biomedical Optics
    • Medical Imaging Technology
    • Optical Physics

    Background:

    • Accurate signal noise floor determination is essential for advanced signal reconstruction in polarization-sensitive optical coherence tomography (PS-OCT).
    • Existing methods for noise floor evaluation in PS-OCT are often subjective and impractical for routine use.
    • Noise floor estimation directly impacts the quality and reliability of quantitative PS-OCT data.

    Purpose of the Study:

    • To develop and validate an automated, objective method for estimating the noise floor in PS-OCT.
    • To improve the accuracy and practicality of noise floor determination for PS-OCT signal processing.
    • To establish a robust noise estimation technique applicable across various PS-OCT systems and in vivo imaging.

    Main Methods:

    • Utilizing the degree of polarization uniformity as a key parameter for noise assessment.
    • Incorporating known speckle intensity statistics to model the noise floor.
    • Developing an automated algorithm for noise floor estimation based on polarization and intensity data.
    • Validating the method through phantom experiments and in vivo imaging studies.

    Main Results:

    • The proposed method accurately models and estimates the PS-OCT noise floor automatically.
    • The noise estimation technique demonstrates robustness across different imaging systems.
    • Successful application of the method in various in vivo imaging scenarios.
    • Objective and reproducible noise floor quantification achieved.

    Conclusions:

    • The developed automated method provides a practical and objective solution for PS-OCT noise floor estimation.
    • This technique enhances the reliability of signal reconstruction and quantitative analysis in PS-OCT.
    • The findings support the broader adoption of accurate noise floor assessment in PS-OCT applications.