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Updated: Aug 6, 2026

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
Published on: May 10, 2022
Immunoreactivity of ubiquitin in human prostate gland
1Department of Human and Clinical Anatomy, Faculty of Medicine and Health Sciences, Sultan Qaboos University, Oman. ziad@just.edu.jo
Background:
Ubiquitin is a low molecular weight protein which has been detected in a variety of normal and cancerous tissues. It is involved in many regulatory processes including protein proteolysis. It has been implicated in tumor pathogenesis. The role of ubiquitin in human prostate gland is investigated in this study.
Methods:
In this study, we utilized immunohistochemistry technique to localize ubiquitin in human prostate gland and correlate it with different pathological conditions of the prostate.
Results:
Ubiquitin was localized in normal, benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma with variations in the distribution and intensity. In BPH, ubiquitin immunoreactivity was localized mainly in the nuclei while in the adenocarcinoma was localized mainly in the cytoplasm.
Conclusion:
The presence of ubiquitin mainly in the nuclei and in the cytoplasm of BPH and prostatic adenocarcinoma, respectively, may suggest a role of ubiquitin in the development of the above mentioned conditions. Ubiquitin could be used as a potential marker for the diagnosis and prognosis of pathological conditions of the prostate.
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