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The normal human amniotic fluid supernatant proteome.

George T Tsangaris1, Ageliki Kolialexi, Panagiotis M Karamessinis

  • 1Division of Biotechnology, Center of Basic Research, Foundation for Biomedical Research of the Academy of Athens, Greece. gthtsangaris@bioacademy.gr

In Vivo (Athens, Greece)
|August 12, 2006
PubMed
Summary

This study constructed a 2D protein map of normal human amniotic fluid supernatant (AFS) using two-dimensional electrophoresis and mass spectrometry. This proteome map aids in identifying potential biomarkers for predicting fetal abnormalities.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Medical Diagnostics

Background:

  • Amniotic fluid (AF) is crucial for prenatal diagnosis.
  • Proteomics aids in identifying disease markers and drug targets.
  • A detailed 2D electrophoresis map of normal human AF supernatant (AFS) was previously unreported.

Purpose of the Study:

  • To construct a comprehensive 2D protein database and map for normal human AFS.
  • To establish a reference proteome for identifying aberrant protein expression.
  • To facilitate the discovery of potential biomarkers for abnormal fetal development.

Main Methods:

  • Proteomic analysis of ten normal human AFS samples.
  • Utilized two-dimensional electrophoresis (2DE) for protein separation.

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  • Employed matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS and MALDI-MS-MS) for protein identification.

    • Main Results:

    • A mean of 412 protein spots were analyzed per gel.
    • A 2D protein map comprising 136 unique gene products was successfully constructed.
    • Identified proteins included regulatory proteins, enzymes, secreted proteins, carriers, and immunoglobulins, along with twelve hypothetical proteins.

    Conclusions:

    • The established normal AFS proteome map serves as a valuable tool for research.
    • It enables the study of aberrant protein expression in fetal abnormalities.
    • This resource aids in the search for novel protein markers for predicting abnormal fetuses.