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SV40: T antigen, the A function and transformation.

M Osborn, K Weber

    Cold Spring Harbor Symposia on Quantitative Biology
    |January 1, 1975
    PubMed
    Summary
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    Simian virus 40 (SV40) T antigen, a nuclear protein, exists in a large complex within transformed cells. This complex may regulate cell DNA synthesis, potentially driving cell transformation.

    Area of Science:

    • Molecular Biology
    • Virology
    • Cell Biology

    Background:

    • Simian virus 40 (SV40) T antigen is a key protein in the nuclei of infected and transformed cells.
    • Understanding T antigen's function is crucial for comprehending viral transformation mechanisms.

    Purpose of the Study:

    • To characterize the physical state and potential function of SV40 T antigen in transformed cells.
    • To investigate the role of T antigen in maintaining cellular transformation and DNA synthesis.

    Main Methods:

    • Purification and characterization of SV40 T antigen.
    • Analysis of T antigen's complex formation within transformed cells.
    • Examination of SV40 mutants affecting T antigen's 'A' function.

    Main Results:

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    • SV40 T antigen exists, at least partly, as a large 22S complex in transformed cells.
    • This 22S complex likely involves T antigen bound to other viral or host proteins.
    • Mutants affecting the 'A' function of T antigen disrupt transformation maintenance and may impact cell cycle progression (G1 to S).

    Conclusions:

    • SV40 T antigen's 22S complex formation suggests a role in regulating cellular processes.
    • The 'A' function of T antigen is likely essential for maintaining transformation, possibly by governing DNA synthesis.
    • SV40 transformation may result from the integration of the viral replicon and its initiator function into the host cell.