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Updated: Aug 6, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
[Dermatohistology laboratory]
M Megahed1, P Kind, J Schaller
1Dermatologisches Labor, Klinik für Dermatologie und Allergologie, Universitätsklinikum der RWTH Aachen, Pauwellsstrasse 30, 52057 Aachen, Deutschland. mmegahed@ukaachen.de
Abstract:
Dermatohistology plays an essential role in the diagnosis of cutaneous tumors and inflammatory skin diseases. In order to practice dermatohistology properly, one needs not only a well-equipped laboratory but also properly trained dermatohistologists and laboratory technicians. We discuss the establishment of a dermatohistology laboratory. The technical procedures involved in making slides, ranging from traditional H & E stains, through special stains, immunohistochemistry and molecular-biological techniques, are viewed. The importance of clinical-histologic correlation and the limitations of pure histologic diagnostics are demonstrated with illustrative cases.

