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Updated: Aug 6, 2026
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
[Detection of rpoB mutations in rifampicin-resistant Mycobacterium kansasii]
Shiomi Yoshida1, Katsuhiro Suzuki, Kazunari Tsuyuguchi
1Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555 Japan. dustin@kch.hosp.go.jp
Purpose:
To detect rifampicin-resistant mutations in Mycobacterium kansasii (M. kansasii).
Methods:
We examined the M. kansasii isolates from sputum of patients at National Hospital Organization Kinki-chuo Chest Medical Center from January 1, 2001 to November 30, 2005 using drug-susceptibility testing, and analyzed 69-bp fragment of rpoB gene in rifampicin-resistant strains.
Results:
Three strains from 314 isolates were determined as rifampicin resistant using drug-susceptibility testing. Those strains showed a rise in minimum inhibitory concentration (MIC), and had the mutations in rpoB gene. These point mutations in codons 513 and 516 were common mutations found in rifampicin-resistant clinical isolates of M. tuberculosis.
Discussion:
We verified the association between rpoB gene mutations and rifampicin resistance in M. kansasii.
