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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
FLP-mediated site-specific recombination for genome modification in turfgrass
Qian Hu1, Kimberly Nelson, Hong Luo
1Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, Clemson, SC 29634, USA.
Biotechnology Letters
|August 17, 2006
Summary
The yeast FLP/FRT system enables controlled gene expression in turfgrass. This technology offers a promising strategy for transgene containment in genetically modified perennial plants.
Area of Science:
- Plant Biotechnology
- Molecular Biology
- Genetics
Background:
- Genetically modified (GM) turfgrass requires effective gene containment strategies.
- Site-specific recombination systems offer precise control over gene expression.
Purpose of the Study:
- To assess the FLP/FRT system's feasibility for controlled genome modification in turfgrass.
- To explore its potential for transgene containment in GM turfgrass.
Main Methods:
- Co-transformation of turfgrass cell cultures (creeping bentgrass and Kentucky bluegrass) with FLP recombinase and a reporter plasmid.
- Utilizing an FRT-flanked blocking sequence to regulate beta-glucuronidase (gusA) gene expression.
- Molecular analysis and functional evaluation in stably transformed creeping bentgrass.
Main Results:
- Successful FLP-mediated excision of the blocking sequence in turfgrass cell cultures.
- Restoration of GUS gene expression following promoter-reporter gene proximity.
- Confirmation of FLP/FRT system functionality in transgenic creeping bentgrass.
Conclusions:
- The FLP/FRT system is a viable tool for genetic manipulation in turfgrass.
- This system holds significant potential for developing transgene containment strategies in perennial plants.
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