Related Experiment Video
Updated: Aug 6, 2026
06:38
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
FBPs are calibrated molecular tools to adjust gene expression
Hye-Jung Chung1, Juhong Liu, Miroslav Dundr
1Laboratory of Pathology, CCR, NCI, Bldg. 10, Rm. 2N106, Bethesda, MD 20892-1500, USA.
Molecular and Cellular Biology
|August 18, 2006
Summary
The FUSE binding protein (FBP) family members show distinct gene regulation functions. Despite unique features, they share targets and mechanisms, acting as a regulatory kit.
Area of Science:
- Molecular Biology
- Gene Regulation
- Protein Interactions
Background:
- The FUSE binding protein (FBP) family comprises three members with diverse attributed functions in gene regulation.
- Investigating sequence differences to determine functional customization versus neutral variation is crucial.
Purpose of the Study:
- To evaluate whether sequence variations in FBP family members correlate with functional specialization.
- To characterize the intrinsic transcription activation/repression, protein binding, and subcellular localization of each FBP.
Main Methods:
- Biochemical assays
- Molecular biology techniques
- Cell biology studies
- Analysis of protein-protein interactions and subcellular localization
Main Results:
- Each FBP exhibited unique profiles in transcription modulation, protein interactions, and trafficking.
- FBP3 showed weakened nuclear localization and unexpectedly failed to bind FBP-interacting repressor (FIR) due to specific sequence substitutions.
- Transactivation and FIR-binding strengths varied inversely among FBPs.
Conclusions:
- FBP family members possess distinguishing features and differential activities.
- Despite variations, FBPs localize to common subnuclear sites and regulate shared target genes like c-myc.
- FBPs function as a molecular regulatory kit, tuning shared target gene expression via a common mechanism.
More Related Videos
Related Concept Videos
02:57Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
02:11Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
02:33Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...