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Microorganism screening for limonene bioconversion and correlation with RAPD markers
Geciane Toniazzo1, Lindomar Lerin, Débora de Oliveira
1Departament of Biochemistry, Instituto de Química - UFRJ, CT, Bloco A, Lab 641 Rio de Janeiro - RJ 21945-970, Brazil.
Applied Biochemistry and Biotechnology
|August 19, 2006
Summary
This study screened microorganisms for limonene biotransformation, identifying four effective strains. However, random amplified polymorphic DNA (RAPD) markers revealed high genetic diversity, showing no correlation with biotransformation efficiency.
Area of Science:
- Biotechnology
- Microbial Biotransformation
- Molecular Biology
Background:
- The biotechnological market is driven by microbial biotransformations of monoterpenes.
- Molecular biology techniques are crucial for efficient strain screening in bioprocesses.
Purpose of the Study:
- To select microbial strains capable of converting limonene via fermentation.
- To utilize random amplified polymorphic DNA (RAPD) markers for strain characterization.
Main Methods:
- Fermentative screening of 17 microbial strains for limonene conversion.
- Application of random amplified polymorphic DNA (RAPD) analysis to assess genetic diversity.
Main Results:
- Four out of 17 tested strains successfully converted limonene into oxygenated derivatives.
- RAPD analysis indicated high genetic polymorphism (96.02%) and low similarity (16.02–51.51%) among strains.
- Significant genetic diversity was observed, even within the same species.
Conclusions:
- Random amplified polymorphic DNA (RAPD) markers are effective in revealing high microbial genetic diversity.
- RAPD markers could not establish a correlation between genetic profiles and limonene biotransformation capabilities.
