[A study of immunologic reactions using biophysical methods]

Chemiluminescent characteristics of the antigen-antibody reaction, effects of gamma-globulin, iodine, hydrogen peroxide, and temperature on the type of the fluorescence of the complex were examined with the use of biomedical chemiluminometer and fluorescent spectrophotometer (Japan). The findings evidence that fluorescence is connected mainly with the tryptophan amino acid residues and with conformational restructuring of protein molecules. Changes in the intensity of chemiluminescence during the formation of a complex may be used for the detection of antibodies in the examinee's body.