Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Live cell refractometry using microfluidic devices.

Niyom Lue1, Gabriel Popescu, Takahiro Ikeda

  • 1G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Optics Letters
|August 29, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collateral damage from violent incidents: human costs of polio immunization.

Bulletin of the World Health Organization·2025
Same author

Tracing chalcolithic population mobility using strontium isotopes and proteomics at Gumelnița site, Romania.

Scientific reports·2025
Same author

Characterization of environmental and clinical surveillance inputs to support prospective integrated modeling of the polio endgame.

PLOS global public health·2025
Same author

Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part II.

Journal of biomedical optics·2024
Same author

Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part I.

Journal of biomedical optics·2024
Same author

Review of Poliovirus Transmission and Economic Modeling to Support Global Polio Eradication: 2020-2024.

Pathogens (Basel, Switzerland)·2024
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Researchers used Hilbert phase microscopy to measure the refractive index of live cells. By confining cells in microchannels, they isolated cell thickness and refractive index for accurate measurements.

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Microscopy

Background:

  • Quantitative phase imaging provides insights into cell properties.
  • Refractive index and thickness are key cellular parameters.
  • Decoupling these parameters is crucial for accurate analysis.

Purpose of the Study:

  • To measure the average refractive index of live cells in culture.
  • To develop a method for separating cell refractive index and thickness contributions to phase signals.
  • To validate the method using controlled cell confinement.

Main Methods:

  • Utilized Hilbert phase microscopy (HPM) for quantitative phase imaging.
  • Employed microchannels to confine cells, controlling their thickness.
  • Measured phase shifts to extract optical path length differences.

Related Experiment Videos

Main Results:

  • Successfully measured the average refractive index of live cells.
  • Demonstrated that microchannel confinement effectively decouples refractive index and thickness.
  • Results were validated against measurements of spherical cells in suspension.

Conclusions:

  • Hilbert phase microscopy is a powerful tool for determining live cell refractive index.
  • Microchannel confinement offers a robust approach to isolate optical properties of cells.
  • This method enhances the accuracy of biophysical measurements on cellular structures.