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Updated: Jul 20, 2026
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Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
Sample preparation project for the subcellular proteome of mouse liver
Yanping Song1, Yunwei Hao, Aihua Sun
1Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing, P R China.
Proteomics
|August 31, 2006
Summary
Subcellular fractionation of mouse liver is optimized for high purity and yield. Frozen liver homogenization offers a method for rare samples, but fresh tissue is best for organelle isolation.
Area of Science:
- Proteomics
- Cell Biology
- Biochemistry
Background:
- Organelle proteome analysis is crucial but challenging.
- Subcellular fractionation requires high purity and yield.
- The Human Liver Proteome Project (HLPP) faces these challenges.
Purpose of the Study:
- To optimize subcellular preparation methods for mouse liver.
- To compare fresh vs. frozen liver homogenization for fractionation.
- To establish an effective method for the HLPP.
Main Methods:
- Developed a method for multiple organelle fractions (plasma membrane, mitochondria, nucleus, ER, cytosol) from a single homogenate.
- Compared fresh, frozen, and frozen homogenized mouse livers.
- Evaluated purity, efficiency, and integrity via protein yield, immunoblotting, and transmission electron microscopy.
Main Results:
- The selected method effectively yields multiple subcellular fractions from mouse liver.
- A selective preparation method for frozen homogenized livers was established.
- Frozen livers are not recommended for optimal organelle isolation compared to fresh livers.
Conclusions:
- The developed method is effective for mouse liver subcellular fractionation.
- A viable method exists for rare clinical samples using frozen homogenized livers.
- Fresh liver tissue is recommended for organelle isolation in proteomic studies, particularly for the HLPP.
