[Efficient plant regeneration in vitro in Pinus massoniana L]

Pinus massoniana L. is one of the important trees for afforestation in South China. The efficient system of plant regeneration from mature zygotic embryos and seedlings of masson pine was established in this study. The influences of basal media, hormones and methods for buds induction, shoots elongation and rooting were studied. The results indicate that DCR medium with 0.5 mg/L BA and 0.05 mg/L IBA shows the highest differentiation rate of adventitious buds. Induction and multiplication of axillary buds take aseptic seedlings as explants. KT has better effect than BA on the axillary buds induction. The best axillary buds induction medium is DCR medium supplemented with 1 mg/L KT and 0.2 mg/L IBA. After culturing on GD medium with 0.1 mg/L BA and 0.2 mg/L IBA for elongation, the buds were transferred on the 1/2 GD medium with 2 mg/L IBA and 0.05 mg/L BA for adventitious roots induction. Paraffin slice indicates that the adventitious buds developed from the meristematic tissue of cotyledonary epicuticula.