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Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: Jul 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

The p53 knowledgebase: an integrated information resource for p53 research.

Y P Lim¹, T T Lim, Y L Chan

¹Bioinformatics Institute, Matrix, Singapore, Singapore.

|September 6, 2006

Summary

The p53 tumor suppressor protein, the guardian of the genome, is crucial for genomic integrity. A new knowledgebase centralizes p53 data to aid cancer research.

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Last Updated: Jul 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Area of Science:

Molecular Biology
Genetics
Cancer Research

Background:

The p53 tumor suppressor protein is vital for genomic stability, regulating cell cycle arrest, senescence, and apoptosis in response to DNA damage.
Mutations in the TP53 gene are frequent in cancer, highlighting p53's critical role as the "guardian of the genome."
A significant body of research exists on p53's function in cancer, necessitating a centralized resource.

Purpose of the Study:

To create a comprehensive, user-friendly knowledgebase for p53-related information.
To facilitate knowledge discovery and research by integrating and curating data on p53.
To provide researchers with accessible tools for analyzing p53 information.

Main Methods:

Initiation of the p53 knowledgebase project (http://p53.bii.a-star.edu.sg).
Development of a web portal with visualization and analysis tools.
Integration of published literature data with manually curated information.

Main Results:

The p53 knowledgebase is a user-friendly portal for accessing curated p53 data.
It includes information on p53 sequence, structure, mutations, polymorphisms, interactions, targets, and modifications.
The portal facilitates knowledge discovery through integrated data and analysis tools.

Conclusions:

The p53 knowledgebase centralizes critical data for researchers studying p53 and cancer.
This resource aims to accelerate research by providing easy access to comprehensive p53 information.
The project supports the scientific community by maintaining and presenting all relevant p53 data.