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Updated: Jul 20, 2026
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
Published on: January 31, 2016
Multicolor PRINS and multicolor PNA
F Pellestor1, P Paulasova, B Andréo
1CNRS UPR 1142, Institute of Human Genetics, Montpellier, France. Franck.Pellestor@igh.cnrs.fr
Abstract:
Both PRimed IN Situ (PRINS) and Peptide Nucleic Acid (PNA) technologies have emerged as research techniques, but they have quickly evolved to applications in biological diagnosis assays. The two procedures now constitute efficient alternatives to the conventional fluorescence in situ hybridization (FISH) procedure for in situ chromosome identification and aneuploidy detection. They present several advantages (specificity, speed, discriminating ability) that make them very attractive for a number of cytogenetic purposes. Multicolor PRINS and PNA protocols have been described for the specific identification of human chromosomes. Various applications have already been developed in human genetics and new adaptations are ongoing.
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