[A study of blood serum proteins using spin probes in athletes during prolonged physical exercise]

Blood serum proteins were studied in walker athletes with the use of a new nitroxyl radical, 4-(phenylethynyl)-2,2,6,6-delta 3-dehydropiperidine-1-oxyl. Methodological aspects are described in detail. The immobilization parameter (IP), calculated from the electroparamagnetic resonance spectrum of the serum with a probe, reflected the distribution of the probe molecules between albumins and lipoproteins (LP), and changes in this parameter values in response to exercise were explained mainly by changed serum protein LP fraction. IP value was regarded as a characteristic of the body reserves connected with endurance phenomenon. The probe used in this work appeared to hold good promise for investigating the body conditions associated with reserve exhaustion, disturbed lipid metabolism, various stress reactions.