Related Experiment Video
Updated: Jul 20, 2026
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
[STR polymorphism of the non-coding human growth hormone gene (HUMGHCSA) and its use in personal identification--a
Magdalena Spólnicka1, Magdalena Konarzewska, Ireneusz Sołtyszewski
1Z Wydziału Biologii Centralnego Laboratorium Kryminalistycznego Komendy Głównej Policji.
Abstract:
The HUMGHCSA locus, located within the non-coding region of the human growth hormone gene on chromosome 17, exhibiting polymorphism of the number of repeats of two and four nucleotide motifs (CT and CTTT), but also polymorphism caused by nucleotide substitution, is one of the most polymorphic STR markers. The purpose of this study was to report the development of a system of fluorescent analysis for an automatic sequencer, to test its reliability and to develop biostatistical parameters of its usefulness in forensic examinations. The studies, carried out on a group of 200 non-related individuals, showed the presence of 24 different alleles, of 221 to 279 bp in length. Biostatistical calculations demonstrated that the expected heterozygosity was 0,898634 +/- 0,015091, PD - 0,980586, PIC - 0,887709, PE - 0,792767, PE for motherless cases - 0,6556701, and the average paternity index - 4,932625. Such results indicate a High usefulness of the studied HUMGHCSA locus for identification examinations.
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