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Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
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Dark immunofluorescence: correlation with serum immunoglobulin abnormalities

J List1, M S Buckland, B Thobhani

  • 1Department of Haematology Immunology Section, Colchester General Hospital, Turner Road, Colchester, Essex CO4 5JL, United Kingdom. jenny.list@essexrivers.nhs.uk

Clinical and Vaccine Immunology : CVI
|September 15, 2006
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...