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Nuclear transfer in nonhuman primates.

Shoukhrat M Mitalipov1, Don P Wolf

  • 1Oregon National Primate Research Center, Beaverton, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2006
PubMed
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Somatic cell nuclear transfer (SCNT) offers a path to genetically identical nonhuman primates for research. While embryonic cell nuclear transfer (NT) is successful in rhesus monkeys, somatic cell cloning remains a challenge.

Area of Science:

  • Reproductive biology
  • Genetics
  • Primate research

Background:

  • Nonhuman primates are crucial models for human diseases.
  • There's a need for genetically specific and identical primates for research, especially in vaccine development and transplantation.
  • Conventional breeding and wild capture cannot meet these demands.

Purpose of the Study:

  • To explore the potential of somatic cell nuclear transfer (SCNT) for producing genetically identical nonhuman primates.
  • To develop efficient SCNT procedures for creating genetically modified primate models of human diseases.
  • To detail protocols for embryonic cell nuclear transfer (NT) in rhesus macaques.

Main Methods:

  • Detailed protocols for rhesus macaque embryonic cell nuclear transfer (NT).

Related Experiment Videos

  • Includes oocyte and embryo production, micromanipulation, and embryo transfer.
  • Focuses on techniques applicable to SCNT.

    • Main Results:

    • Successful production of monkeys using NT with embryonic blastomeres.
    • Demonstrated high in vitro development rates of embryos comparable to in vitro fertilization controls.
    • Somatic cell cloning in primates has not yet been achieved due to limited developmental potential of SCNT embryos.

    Conclusions:

    • Basic nuclear transfer procedures are viable for producing nonhuman primate embryos.
    • SCNT holds promise for generating genetically modified primates and disease models.
    • Further modifications to SCNT protocols are necessary for successful primate cloning from somatic cells.