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Evaluation of intracellular lipids by standardized staining with a Sudan black B fraction
1Biophysics Unit, Tata Memorial Centre, Parel, Bombay, India.
Abstract:
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography. The cytophotometric assessment of intracellular neutral lipids, stained with SBB-I, is described and applied to the study of changes in granulocytic neutral lipids in leukemia.
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