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Related Experiment Videos

[Solid-phase immunoenzyme analysis of apoprotein A-1].

L M Poliakov, O N Poteriaeva, L E Panin

    Laboratornoe Delo
    |January 1, 1990
    PubMed
    Summary

    This study optimized an immunoassay to measure apolipoprotein A-1 (apoA-1) in human serum. The optimized method accurately quantifies apoA-1 levels, establishing normal ranges for males and females.

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    Activity of matrix metalloproteinases during antimycobacterial therapy in mice with simulated tuberculous inflammation.

    Bulletin of experimental biology and medicine·2013

    Area of Science:

    • Biochemistry
    • Immunology

    Context:

    • Apolipoprotein A-1 (apoA-1) is a key component of high-density lipoprotein (HDL) and plays a crucial role in reverse cholesterol transport.
    • Accurate measurement of apoA-1 levels is important for assessing cardiovascular disease risk.

    Purpose:

    • To optimize an indirect solid-phase enzyme immunoassay for the quantitative measurement of human blood serum apoA-1.
    • To establish reliable working conditions for assay components and conjugates.
    • To investigate the impact of serum pretreatment on the detection of apoA-1 antigenic determinants.

    Summary:

    • An indirect solid-phase enzyme immunoassay was optimized for human serum apoA-1 measurement.
    • Immunochemical identification and selection of optimal working regimens for apoA-1, antibodies, and conjugates were performed.
    • The relationship between serum pretreatment methods and the detection of apoA-1 antigenic determinants was established.
    • Normal serum apoA-1 levels were determined as 1.06 +/- 0.1 g/l for females and 1.05 +/- 0.1 g/l for males.

    Impact:

    • Provides a validated and optimized immunoassay for routine clinical assessment of apoA-1 levels.
    • Contributes to a better understanding of apoA-1 quantification and its relationship with serum processing.
    • Establishes reference ranges for apoA-1, aiding in the diagnosis and management of dyslipidemias and cardiovascular health.

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