Related Experiment Video
Updated: Feb 10, 2026

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
Published on: November 4, 2014
[Determination of the concentration of fructose-2,6-bisphosphate using phosphofructokinase]
Abstract:
Measurement of fructose-2,6-bisphosphate (F-2,6-P2) level in biologic material is based on its ability to eliminate allosteric inhibition of phosphofructokinase (PFK) with high ATP concentrations. Effects of the buffer types and length of storage of commercial PFK from rabbit muscle, manufactured by Boehringer, on the enzyme ability to inhibit ATP and activate F-2,6-P2 were examined. The enzyme showed the highest sensitivity to ATP inhibition in tris buffer and the least one in morpholine propane sulfonic acid. If PFK is stored for a year its sensitivity to ATP inhibition and F-2,6-P2 activation essentially reduces and it becomes unfit for measurement of F-2,6-P2 concentration in biologic material.
More Related Videos
Related Concept Videos
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Energy-requiring Steps of Glycolysis
Concentration Cells
Consider the following voltaic cell:
Calculating Equilibrium Concentrations
A more...
Concentration and Rate Law
For example, in a generic reaction aA + bB ⟶ products, where a and b are stoichiometric coefficients, the rate law can be written as:
Solution Concentration and Dilution

