Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Axon voltage-clamp simulations. III. Postsynaptic region.

R W Joyner, J W Moore, F Ramón

    Biophysical Journal
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hippocampal microstructural changes following electroconvulsive therapy in severe depression.

    Molecular psychiatry·2025
    Same author

    Investigating the effect of cognitive load on the intentionality bias.

    Psychological research·2024
    Same author

    The impact of preoperative sarcopenia on postoperative ileus following colorectal cancer surgery.

    Techniques in coloproctology·2023
    Same author

    Manipulations of Libet clock parameters affect intention timing awareness.

    Scientific reports·2022
    Same author

    Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.

    Techniques in coloproctology·2022
    Same author

    Impact of STIMUlant and osmotic LAXatives (STIMULAX trial) on gastrointestinal recovery after colorectal surgery: randomized clinical trial.

    The British journal of surgery·2021

    Numerical simulations show that while voltage clamping excitable cells with oil gaps can accurately determine sodium equilibrium potential (ENa), errors in synaptic reversal potential (ES) are significant. Blocking potassium conductance improves ES accuracy.

    Area of Science:

    • Neuroscience
    • Computational Biology
    • Biophysics

    Background:

    • The voltage clamp technique is crucial for studying excitable cells.
    • Accurate measurements of synaptic potentials are essential for understanding neuronal function.
    • Previous methods for voltage clamping specific cell regions had limitations.

    Purpose of the Study:

    • To numerically simulate and evaluate a new voltage clamp technique using oil drops for insulating synaptic regions.
    • To assess the accuracy of determining sodium equilibrium potential (ENa) and synaptic reversal potential (ES) with this method.
    • To investigate the impact of oil gap length, electrode placement, and ion channel blockers on measurement accuracy.

    Main Methods:

    • Numerical simulations of an excitable cylindrical cell model.

    Related Experiment Videos

  • Modeling the application of oil drops to insulate a postsynaptic region.
  • Simulating voltage clamp experiments with variable electrode positions.
  • Analyzing the effects of tetraethylammonium chloride (TEA) on synaptic conductance.

    • Main Results:

    • Accurate determination of ENa is achievable with feasible oil gap lengths.
    • Significant errors in ES determination were observed, dependent on membrane resistance.
    • Blocking potassium conductance with TEA substantially reduced errors in ES.
    • Cable length and electrode position influence the apparent synaptic conductance.

    Conclusions:

    • The simulated oil gap technique shows promise for voltage clamping excitable cells, particularly those with nonsomatic synapses.
    • Careful consideration of experimental parameters is needed to minimize errors in ES.
    • Pharmacological manipulation, like using TEA, can enhance the accuracy of synaptic potential measurements.