Related Experiment Videos
Wound healing angiogenesis: indirect stimulation by basic fibroblast growth factor
D R Knighton1, G D Phillips, V D Fiegel
1Department of Surgery, University of Minnesota, Minneapolis 55455.
The Journal of Trauma
|December 1, 1990
Summary
Basic fibroblast growth factor (bFGF) delivered in Elvax stimulated angiogenesis indirectly via inflammation. However, bFGF in Hydron did not directly promote blood vessel growth, suggesting delivery method is crucial for therapeutic effects.
Area of Science:
- Ophthalmology
- Biomaterials Science
- Vascular Biology
Background:
- Basic fibroblast growth factor (bFGF) is known to promote endothelial cell proliferation.
- Understanding the in vivo angiogenic potential of bFGF is critical for therapeutic applications.
Purpose of the Study:
- To evaluate the ability of basic fibroblast growth factor (bFGF) to stimulate angiogenesis in vivo.
- To compare the effects of bFGF delivery via Hydron and Elvax polymers in a rabbit corneal model.
Main Methods:
- Basic fibroblast growth factor (bFGF) was incorporated into Hydron and Elvax polymers at varying concentrations.
- Polymers were implanted into rabbit corneas, and angiogenesis was assessed over 7 days using a graded scale and histology.
- Human serum albumin (HSA) and transforming growth factor-beta (TGF-beta) served as negative and positive controls, respectively.
Main Results:
- bFGF in Elvax induced significant inflammation-associated angiogenesis (+3 to +4) at all tested doses.
- bFGF in Hydron showed no significant angiogenesis at lower doses, but induced inflammation and angiogenesis at 1,000 ng.
- TGF-beta induced robust angiogenesis with inflammation, while HSA showed no effect.
Conclusions:
- The combination of bFGF and Elvax stimulates angiogenesis indirectly through an inflammatory response.
- bFGF delivered in Hydron did not directly induce angiogenesis or inflammation at comparable concentrations.
- These findings suggest that bFGF may not directly stimulate in vivo angiogenesis, and its effect is dependent on the delivery vehicle and associated inflammation.