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[Expression of neuropilin-1 in human laryngeal carcinoma and cell lines]
1Department of Otolaryngology Study, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
Objective:
To study the expression of Neuropilin-1 in human carcinoma and papilloma of larynx specimens and carcinoma cell lines Hep-2.
Method:
The expression of Neuropilin-1 in human laryngeal carcinoma, papilloma specimens were investigated by immunohistochemical staining; the expression of Neuropilin-1 mRNA in human laryngeal squamous cell carcinoma, papilloma specimens and cell lines were examined by using RT-PCR.
Result:
Immunohistochemical analysis revealed that NRP-1 was expressed in all human laryngeal squamous cell carcinoma specimens but was absent in nonmalignant laryngeal tissue. The mRNA of NRP land Hep-2 cell lines are positive in 24 of 28 human laryngeal carcinoma specimens but not negative in nonmalignant laryngeal specimens.
Conclusion:
Neuropilin-1 was expressed in most of human laryngeal carcinoma specimens and cell lines but not expressed in nonmalignant tissue. It suggests that the expression of Neuropilin-1 may be related to the risk and development of laryngeal carcinoma and has high distinguishability.
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