Related Experiment Video
Updated: Jul 19, 2026
Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
Isolation, molecular cloning and in vitro expression of rhesus monkey (Macaca mulatta) prominin-1.s1 complementary
S M Husain1, Y Shou, B P Sorrentino
1Division of Stem Cell Transplantation, Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. syed.husain@stjude.org
Abstract:
Human prominin-1 (CD133 or AC133) is an important cell surface marker used to isolate primitive hematopoietic stem cells. The commercially available antibody to human prominin-1 does not recognize rhesus prominin-1. Therefore, we isolated, cloned and characterized the complementary DNA (cDNA) of rhesus prominin-1 gene and determined its coding potential. Following the nomenclature of prominin family of genes, we named this cDNA as rhesus prominin-1.s1. The amino acid sequence data of the putative rhesus prominin-1.s1 could be used in designing antigenic peptides to raise antibodies for use in isolation of pure populations of rhesus prominin-1(+) hematopoietic cells. To the best of our knowledge, there has been no previously published report about the isolation of a prominin-1 cDNA from rhesus monkey (Macaca mulatta).
