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Updated: Jul 19, 2026
10:17
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
Published on: April 29, 2022
Mitotic phosphatases: no longer silent partners.
Laura Trinkle-Mulcahy1, Angus I Lamond
1Division of Gene Regulation & Expression, School of Life Sciences, University of Dundee, Dow St., Dundee DD1 5EH, UK. l.trinklemulcahy@dundee.ac.uk
Current Opinion in Cell Biology
|October 13, 2006
Summary
Protein phosphatases, including PP1, PP2A, CDC25, and Cdc14, are crucial regulators of cell division. Recent studies reveal their roles in sister chromatid cohesion, chromosome segregation, and DNA damage checkpoints during mitosis.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Protein phosphatases are key regulators of cellular processes.
- Mitosis involves complex regulatory networks controlling cell division.
- Specific phosphatases like PP1, PP2A, CDC25, and Cdc14 have been implicated in cell cycle control.
Purpose of the Study:
- To summarize recent advances in understanding the roles of protein phosphatases in mitosis.
- To highlight the diverse functions of PP1, PP2A, CDC25, and Cdc14 in cell division.
- To emphasize the active regulatory role of phosphatases in cell cycle control.
Main Methods:
- Review of recent scientific literature on protein phosphatases and mitosis.
- Analysis of studies defining the functions of PP1, PP2A, CDC25, and Cdc14.
- Integration of findings on phosphatase interactions and regulatory mechanisms.
Main Results:
- PP2A regulates sister chromatid cohesion via shugoshin interaction.
- PP1's chromatin targeting subunit is essential for chromosome segregation.
- CDC25 activity is linked to cyclin-dependent kinase activation and DNA damage checkpoints.
- Cdc14 has novel roles in rDNA, telomere segregation, and spindle assembly.
Conclusions:
- Protein phosphatases play active and diverse roles in regulating mitosis.
- These phosphatases are critical for accurate chromosome segregation and cell cycle progression.
- Understanding phosphatase function provides insights into cell division control.
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