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Published on: September 27, 2018
[Application of optical proteinchip in detecting phage M13KO7]
Cai Qi1, Jing Feng, Zhan-Hui Wang
1Institute of Mechanics, Chinese Academy of Sciences, Beijing, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 14, 2006
Summary
This study developed a label-free optical biosensor for detecting M13 bacteriophage. The biosensor utilizes antibody immobilization and imaging ellipsometry, achieving high sensitivity for viral detection.
Area of Science:
- Biotechnology
- Biosensor Technology
- Surface Chemistry
Context:
- Developing sensitive and rapid detection methods for viruses is crucial for public health.
- Existing viral detection methods can be time-consuming and require complex labeling procedures.
- Optical biosensors offer potential for label-free, real-time viral detection.
Purpose:
- To develop and validate an optical protein-chip for detecting M13 bacteriophage (M13KO7).
- To immobilize a specific antibody (GP3) onto a silicon wafer surface for phage capture.
- To quantify phage concentration using imaging ellipsometry based on layer thickness variations.
Summary:
- Avidin-biotin interaction was used to immobilize biotinylated anti-M13 antibody (GP3) on aldehyde-modified silicon wafers.
- The antibody-functionalized surface captured M13KO7 phage, causing a detectable change in layer thickness measured by imaging ellipsometry.
- The system demonstrated a sensitivity of 10^9 plaque-forming units/mL for phage detection within a concentration range of 0.1 x 10^10 to 2.5 x 10^10 pfu/mL.
Impact:
- This label-free, quantitative, and visual detection method offers a short measurement time compared to traditional techniques.
- The developed biosensor shows significant potential for investigating antibody-virus interactions.
- It holds promise for the early diagnosis of virosis (viral infections).
