[Research on extracting process for compound shenzhutiaozhi capsule]

Area of Science:

• Pharmacognosy
• Natural Product Chemistry
• Traditional Chinese Medicine Formulation

Background:

• Shenzhutiaozhi capsule is a compound herbal medicine.
• Optimizing extraction is crucial for maximizing the therapeutic potential of herbal formulations.
• Key active compounds include oleanolic acid and salvianolic acid B.

Purpose of the Study:

• To investigate and determine the optimal extraction techniques for the compound Shenzhutiaozhi capsule.
• To identify the best methods for extracting oleanolic acid and salvianolic acid B.

Main Methods:

• Orthogonal experimental design was employed to systematically optimize extraction parameters.
• Thin-Layer Chromatography (TLC) scanning was used to detect oleanolic acid.
• High-Performance Liquid Chromatography (HPLC) was utilized for the quantification of salvianolic acid B.

Main Results:

• Optimal extraction conditions were determined for Fructus Ligustri lucid, Salvia miltiorrhizae, and Rhizoma Atractylodis Macrocephalae.
• Specific parameters include using five volumes of 70% alcohol for Fructus Ligustri lucid and seven volumes of water for Salvia miltiorrhizae and Rhizoma Atractylodis Macrocephalae.
• Each extraction was performed three times for 30 minutes.

Conclusions:

• The established extraction techniques significantly improve the extraction rates of active components.
• This optimization ensures a higher yield of oleanolic acid and salvianolic acid B.
• The findings support the efficient production of Shenzhutiaozhi capsule with enhanced therapeutic value.