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Updated: Aug 19, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
[The determination of free hemoglobin in blood plasma using the hemiglobincyanide method]
Abstract:
The authors have proved the possibility of using the hemiglobincyanide method for measurements of blood plasma free hemoglobin: free hemoglobin was measured in autoblood reinfused from the operation field by various methods (45-benzidine method, 3 parallel analyses in every study, and 45-hemiglobincyanide method, modified by increasing the amount of the tested material from 0.02 to 2.0 ml, also 3 parallel analyses per sample); a correction coefficient equal to 71.7 is suggested, and correction of the data according to a formula.
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