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Updated: Jul 19, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Basidiomycete cultures on perlite survive successfully repeated freezing and thawing in cryovials without
Ladislav Homolka1, Ludmila Lisá, Frantisek Nerud
1Institute of Microbiology AS CR, Vídenská 1083, 142 20 Prague 4, Czech Republic. homolka@biomed.cas.cz
Abstract:
Mycelial basidiomycete cultures on perlite in cryovials survived successfully three successive cycles of freezing, storage in liquid nitrogen (LN) and thawing without noticeable changes. This indicates that using perlite as a carrier for cryopreservation could in most cases overcome difficulties caused by interrupted supply of LN or electric power during the storage. Cultures on perlite can also be reused for successive inoculations.

