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Related Experiment Videos

Plasma membrane IP3 receptors.

C W Taylor1, O Dellis

  • 1Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK. cwt1000@cam.ac.uk

Biochemical Society Transactions
|October 21, 2006
PubMed
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Inositol 1,4,5-trisphosphate receptors (IP3Rs) function in the plasma membrane, mediating significant calcium (Ca2+) entry in DT40 cells. This Ca2+ influx via IP3Rs has distinct cellular effects compared to store-operated channels.

Area of Science:

  • Cellular Biology
  • Molecular Physiology
  • Ion Channel Function

Background:

  • Inositol 1,4,5-trisphosphate receptors (IP3Rs) are primarily known for their role in intracellular calcium signaling.
  • Their presence and function within the plasma membrane of animal cells remain largely uncharacterized.

Purpose of the Study:

  • To investigate the presence and functional role of IP3Rs in the plasma membrane.
  • To determine the contribution of plasma membrane IP3Rs to calcium (Ca2+) entry in DT40 cells.

Main Methods:

  • Whole-cell patch-clamp electrophysiology was employed on DT40 cells.
  • Studies utilized cells expressing native or mutated forms of IP3Rs.

Main Results:

  • DT40 cells express a small number (two to three) of functional IP3Rs in their plasma membrane.

Related Experiment Videos

  • Approximately 50% of B-cell receptor-stimulated Ca2+ entry is mediated by these plasma membrane IP3Rs, not solely by store-operated Ca2+ entry.
  • Ca2+ entry through the few large-conductance plasma membrane IP3Rs differs functionally from entry via numerous low-conductance store-operated channels.

    • Conclusions:

    • Plasma membrane IP3Rs are significant contributors to cellular calcium homeostasis.
    • The distinct conductance and number of these channels suggest unique roles in cellular signaling pathways.