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Updated: Jul 19, 2026
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
[Construction and preliminary application of a human natural phage antibody library with diversity]
Jin Wang1, Wen-Xin Luo, Li-Feng Li
1The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Research Center for Medical Molecular Virology of Fujian Province, Xiamen 361005, China. wxluo@jingxian.xmu.edu.cn
Aim:
To construct a human natural phage single-chain antibody (scFv) library with diversity.
Methods:
V(H) and V(L) genes were amplified by RT-PCR and hemi-PCR from peripheral blood lymphocytes of healthy persons. The V genes were assembled to form scFv by overlap PCR and cloned into phagemid pCANTAB-5E, and then transformed into E. coli TG1 by electroporation to construct a human natural phage scFv library. The diversity and gene family of antibody gene were analysed by sequencing and the specific antibodies against various antigens were screened through bio-panning.
Results:
A human natural phage scFv library with diversity and 2x10(8) sink size was constructed successfully. The specific human scFvs against 5 antigens were obtained by bio-panning.
Conclusion:
A human natural phage scFv library with diversity is constructed successfully and can be applied to human antibody preparation.
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