Related Experiment Video
Updated: Jul 18, 2026
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
Published on: February 23, 2019
[Effects of sodium arsenite on catalase in human keratinocytes]
Xian-ce Sun1, Feng-yuan Piao, Yi Wang
1Hygiene Department, Colleges of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116027, China.
Objective:
To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).
Methods:
The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.
Results:
If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).
Conclusion:
CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.
