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[The use of lyophilized plasma as a reference sample for determining factor VIII]
Laboratornoe Delo
|January 1, 1991
Summary
Lyophilized Soviet reference plasma Factor VIII showed 55-135% activity over 18-43.5 months. These findings suggest its potential use as a reliable reference reagent for Factor VIII assays.
Area of Science:
- Hematology
- Biochemistry
- Clinical Diagnostics
Context:
- Accurate measurement of Factor VIII is crucial for diagnosing and managing hemophilia.
- Standardized reference materials are essential for reliable laboratory testing.
- Lyophilized plasma offers advantages in stability and storage for reference reagents.
Purpose:
- To evaluate the stability and activity of lyophilized Soviet reference plasma Factor VIII.
- To assess its suitability as a reference reagent for Factor VIII activity assays.
- To identify potential sources of variability in Factor VIII measurements.
Summary:
- Lyophilized Soviet reference plasma Factor VIII was assayed using the Papayan and Khrolova method.
- Factor VIII activity ranged from 55% to 135% after storage for 18-43.5 months.
- Variability in parallel assays was attributed to non-standard native controls, highlighting the need for standardized methods.
Impact:
- The study indicates promising potential for using lyophilized donor plasma as a reference standard.
- This could improve the accuracy and consistency of Factor VIII activity estimations.
- Standardization of reference reagents will enhance diagnostic reliability in hemophilia management.