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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jul 18, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

ZBP1 regulates mRNA stability during cellular stress.

Nadine Stöhr1, Marcell Lederer, Claudia Reinke

  • 1NBL3 Research Group, Zentrum für Angewandte Medizinische und Humanbiologische Forschung, Department of Medicine, Martin-Luther-University, 06120 Halle, Germany.

The Journal of Cell Biology
|November 15, 2006
PubMed
Summary

Zipcode-binding protein 1 (ZBP1) prevents mRNA degradation during cellular stress. This protein stabilizes specific mRNAs within stress granules, ensuring proper translational adaptation and preventing premature decay.

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Measurements of Physiological Stress Responses in C. Elegans
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Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Related Experiment Videos

Last Updated: Jul 18, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The integrated stress response (ISR) involves reversible translational suppression.
  • mRNA silencing occurs in cytoplasmic stress granules (SGs), which associate with processing bodies (PBs).
  • Mechanisms protecting mRNAs from degradation within SGs are not fully understood.

Purpose of the Study:

  • To investigate the role of Zipcode-binding protein 1 (ZBP1) in mRNA regulation during cellular stress.
  • To determine how ZBP1 influences mRNA turnover and stability within stress granules.

Main Methods:

  • Investigated ZBP1's function in mRNA fate regulation in unstressed and stressed cells.
  • Assessed the impact of ZBP1 knockdown and overexpression on target mRNA stability during ISR.
  • Analyzed ZBP1's association with mRNAs in stress granules.

Main Results:

  • ZBP1 regulates the cytoplasmic fate of specific mRNAs.
  • ZBP1 association with mRNAs in SGs is not required for SG targeting.
  • ZBP1 knockdown selectively destabilizes target mRNAs during ISR; overexpression enhances stability.

Conclusions:

  • mRNA stabilization during cellular stress requires specific protein-mRNA interactions, not just SG targeting.
  • ZBP1 retains mRNAs in SGs, preventing premature decay in PBs.
  • mRNA-binding proteins like ZBP1 are crucial for translational adaptation during stress by modulating mRNA turnover.