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Immobilized metal ion affinity partitioning, a method combining metal-protein interaction and partitioning of
G Birkenmeier1, M A Vijayalakshmi, T Stigbrand
1Institute of Biochemistry, Karl-Marx-Universität Leipzig, Germany.
Abstract:
Immobilized metal ions were used for the affinity extraction of proteins in aqueous two-phase systems composed of polyethylene glycol (PEG) and dextran or PEG and salt. Soluble chelating polymers were prepared by covalent attachment of metal-chelating groups to PEG. The effect on the partitioning of proteins of such chelating PEG derivatives coordinated with different metal ions is demonstrated. The proteins studied were alpha 2-macroglobulin, tissue plasminogen activator, superoxide dismutase and monoclonal antibodies. The results indicate that immobilized metal ion affinity partitioning provides excellent potential for the extraction of proteins.