Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 18, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Tissue factor and thrombomodulin expression on endothelial cell-seeded collagen modules for tissue engineering.

Mingyu She1, Alison P McGuigan, Michael V Sefton

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada.

Journal of Biomedical Materials Research. Part A
|November 18, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering in vitro models to target tumour recurrence.

Trends in biotechnology·2026
Same author

Synthetic living materials in cancer biology.

Nature reviews bioengineering·2026
Same author

Tellurophene-Tagged Carfilzomib Enables Single-Cell Mass Cytometric Mapping of Proteasome Activity.

ACS chemical biology·2025
Same author

Regenerative Healing.

Tissue engineering. Part A·2025
Same author

Correction: Analysis of an engineered organoid model of pancreatic cancer identifies hypoxia as a contributing factor in determining transcriptional subtypes.

Scientific reports·2025
Same author

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform.

Journal of visualized experiments : JoVE·2025

Tissue engineering constructs require non-thrombogenic endothelial cells. Human umbilical vein endothelial cells on collagen gel modules showed low thrombogenicity markers, essential for vascular network mimicry in engineered tissues.

Area of Science:

  • Biomaterials Science
  • Vascular Biology
  • Tissue Engineering

Background:

  • Functional tissue engineering necessitates constructs with well-formed vasculature.
  • Endothelial cells lining vascular beds must exhibit a non-thrombogenic phenotype for successful integration.
  • A modular approach to tissue engineering involves assembling smaller, pre-fabricated components.

Purpose of the Study:

  • To assess the non-thrombogenic potential of human umbilical vein endothelial cells (HUVECs) seeded on collagen gel modules.
  • To evaluate the expression of key thrombogenicity markers (tissue factor and thrombomodulin) on HUVECs in a static, modular construct environment.
  • To determine if HUVECs maintain a non-thrombogenic phenotype under conditions mimicking early-stage engineered vascular networks.

Main Methods:

More Related Videos

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

Related Experiment Videos

Last Updated: Jul 18, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

  • Confocal microscopy was employed to visualize HUVEC morphology and distribution on collagen gel modules.
  • Immunofluorescence staining was used to quantify the expression of tissue factor and thrombomodulin.
  • HUVECs were seeded on collagen gel modules and cultured under static conditions for 2 and 7 days.
  • Tumor Necrosis Factor alpha (TNF-alpha) stimulation was used to assess cellular response to inflammatory stimuli.

Main Results:

  • HUVECs rapidly covered the collagen gel modules, forming tight cell-cell junctions and exhibiting cobblestone morphology within 2 days.
  • A low basal expression of tissue factor was observed, which significantly increased upon TNF-alpha stimulation.
  • Thrombomodulin was highly expressed by HUVECs, and this expression decreased following TNF-alpha stimulation.
  • These findings indicate a generally non-thrombogenic phenotype in HUVECs on modules without flow.

Conclusions:

  • Human umbilical vein endothelial cells seeded on collagen gel modules demonstrate markers of low thrombogenicity in a static environment.
  • The observed expression patterns of tissue factor and thrombomodulin suggest suitability for creating vascularized tissue engineering constructs.
  • This modular approach, utilizing endothelialized components, shows promise for developing functional engineered tissues with integrated vascular networks.