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Published on: September 5, 2012
Determination of glucuronidated 5-hydroxytryptophol (GTOL), a marker of recent alcohol intake, by ELISA technique
Jutta Dierkes1, Manfred Wolfersdorf, Katrin Borucki
1Institute of Clinical Chemistry, Otto von Guericke University, Magdeburg, Germany.
Objectives:
To compare markers of alcohol consumption.
Design And Methods:
Measurement of urinary ethyl glucuronide, 5-hydroxytryptophol, 5-hydroxytryptophol glucuronide, 5-hydroxyindole acetic acid in 10 patients during alcohol withdrawal.
Results:
5-Hydroxytryptophol glucuronide was measured by ELISA with good analytical precision, its diagnostic specificity and sensitivity was better than that of 5-hydroxytryptophol and its correlation was closer to ethyl glucuronide than to 5-hydroxytryptophol.
Conclusion:
Determination of 5-hydroxytryptophol glucuronide by ELISA offers promising results in detection of previous alcohol consumption.
