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Automated sample treatment by flow techniques prior to liquid-phase separations.

Georgios A Theodoridis¹, Constantinos K Zacharis, Anastasios N Voulgaropoulos

¹Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. gtheodor@chem.auth.gr <gtheodor@chem.auth.gr>

Journal of Biochemical and Biophysical Methods

|November 23, 2006

Summary

Automated on-line sample preparation using flow techniques enhances analytical processes. This review covers trends, strategies, and applications for miniaturized, automated sample enrichment prior to separation.

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Area of Science:

Analytical Chemistry
Separation Science

Background:

Sample preparation (SP) is crucial for analytical processes.
There is growing interest in novel, advanced SP technologies focusing on miniaturization, automation, and enrichment.

Purpose of the Study:

To review current trends in on-line automated sample preparation using flow-through techniques.
To describe strategies and interfaces for coupling flow techniques with liquid-phase separations.
To highlight applications of these coupled systems.

Main Methods:

Review of literature on flow-through techniques for on-line sample preparation.
Description of strategies for coupling flow techniques with liquid chromatography, capillary electrophoresis, and mass spectrometry.
Discussion of advantages and limitations of coupled systems.

Main Results:

Flow techniques offer effective on-line automated sample preparation.
Various strategies and interfaces exist for coupling flow techniques with separation methods.
Coupled systems demonstrate advantages in miniaturization, automation, and enrichment.

Conclusions:

On-line automated sample preparation by flow techniques is a significant trend in analytical chemistry.
Coupling flow techniques with separation methods like LC, CE, and MS offers efficient solutions for modern analytical challenges.
Further development in this area promises enhanced analytical performance.