Multisample preparation methods for the solvent-free MALDI-MS analysis of synthetic polymers

Area of Science:

• Analytical Chemistry
• Polymer Science
• Mass Spectrometry

Background:

• Current solvent-free matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) methods are limited to single-sample preparation and transfer.
• This limitation hinders high-throughput analysis of synthetic polymers.

Purpose of the Study:

• To develop and present a novel approach for simultaneous, solvent-free sample preparation and automated transfer for MALDI MS.
• To improve efficiency, reduce sample consumption, and minimize cross-contamination in polymer analysis.

Main Methods:

• Development of a multiplexed sample preparation technique using a 384-well plate.
• Simultaneous homogenization of multiple samples via a common laboratory vortex device.
• Integration of automated sample transfer to the MALDI target plate.

Main Results:

• Successful preparation and analysis of up to 384 samples simultaneously.
• Demonstrated high-quality mass spectra for synthetic polymer standards up to 10 kDa.
• Reduced sample and matrix consumption, with a non-critical sample/salt/matrix ratio.
• Homogenization time reduced to under 5 minutes for multiple samples.

Conclusions:

• The novel approach effectively combines simultaneous solvent-free sample preparation with automated transfer for MALDI MS.
• This method significantly enhances efficiency, reduces resource requirements, and minimizes cross-contamination.
• The technique provides a practical and high-throughput solution for the MALDI MS analysis of synthetic polymers.