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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Sequencing single molecules of DNA.
1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK. hagan.bayley@chem.ox.ac.uk
Current Opinion in Chemical Biology
|November 23, 2006
Summary
The National Institutes of Health aimed for a 1,000 dollars human genome by 2015. Current sequencing technologies are unlikely to meet this goal, with single-molecule approaches showing the most promise for rapid, low-cost sequencing.
Area of Science:
- Genomics
- Biotechnology
- Molecular Biology
Background:
- The National Institutes of Health (NIH) initiated the 1,000 dollars genome challenge in 2004.
- The objective was to achieve rapid and affordable sequencing of a complete human genome by 2015, with an interim goal of 100,000 dollars genome by 2010.
Purpose of the Study:
- To evaluate the feasibility of achieving the 1,000 dollars genome goal.
- To assess the potential of different sequencing technologies in reducing genome sequencing costs.
Main Methods:
- Review of existing sequencing technologies, including Sanger sequencing and emerging massively parallel technologies.
- Analysis of cost reduction trends and future potential of various sequencing approaches.
Main Results:
- Sanger sequencing, despite significant cost reductions, is unlikely to achieve the 100,000 dollars genome target.
- Massively parallel sequencing technologies are progressing towards lower costs but may not reach the 1,000 dollars genome goal.
- Single-molecule sequencing approaches are identified as the most promising for ultrarapid and low-cost genome sequencing.
Conclusions:
- Achieving the 1,000 dollars genome requires advancements beyond current Sanger and massively parallel sequencing methods.
- Single-molecule technologies represent the most viable path towards realizing the ambitious goal of affordable whole human genome sequencing.
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